Complement C5 (C5) Mouse Monoclonal Antibody [Clone ID: 557]

Specifications

Product Data
Clone Name	557
Applications	ELISA, FN, WB
Recommend Dilution	Functional assays: Product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Immunoassays (1,2,3): Detection antibody in ELISA. (Ref.1). Western blot (1): Used as ascites at a 1/1000 dilution. Incubation 2h on nitrocellulose blotted samples. (Ref.1). Positive control: Recombinant C5. Negative control: Recombinant C5.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human C5
Specificity	The monoclonal antibody 557 recognizes an epitope of complement factor 5 (C5) and C5a. It is capable to inhibit the binding of C5a to the C5a receptor through a competitive mechanism, it does not block the cleavage of C5 into C5a and C5b.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	complement component 5
Database Link	Entrez Gene 727 Human UniProt ID P01031
Background	The complement system is composed of over 30 proteins, activated in response to tissue injury, invading pathogens or other foreign surfaces. The complement pathways can be divided in the activation pathways and lytic pathway. The activation pathways lead via C3 to the cleavage of the fifth complement component C5. C5a was first described as a cleavage product of C5 with chemotactic and anaphylatoxic properties. Further characterization revealed that C5a is an essential part of the innate immune response and evidence now suggests that it may also play a role in adaptive immunity. Complement fragment C5a is a 74 residue pro-inflammatory polypeptide. C5a induces smooth muscle contraction, increases vascular permeability, causes degranulation of mast cells and basophils, and release of lysosomal enzymes. In addition C5a stimulates the directed migration of neutrophils, eosinophils, basophils and monocytes. C5a binds to at least two seven-transmembrane domain receptors, C5aR (C5R1, CD88) and C5L2 (gpr77), expressed ubiquitously on a wide variety of cells but particularly on the surface of immune cells like macrophages, neutrophils and T cells. The former is a well-established receptor that initiates G-protein-coupled signaling via mitogen-activated protein kinase pathways, thereby by inducing synthesis of cytokines such as TNF-alpha, IL-1beta,  IL-6 and IL-8. Its in vivo blockade greatly reduces inflammatory injury. Much less is known about C5L2, occupancy of which by C5a does not initiate increased intracellular Ca(2+).The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases. Nowadays C5a is also implicated in non-immunological functions associated with developmental biology, CNS development and neurodegeneration, tissue regeneration, and haematopoiesis.
Synonyms	CPAMD4, Complement component 5
Reference Data

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