Lipoteichoic Acid (LTA) Mouse Monoclonal Antibody [Clone ID: 55]

Specifications

Product Data
Clone Name	55
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections: The typical starting working dilution is 1/50. Flow Cytometry: 1/250 (Ref.10). Immunoassay: For detection, 1.2 µg/ml antibody in PBS was added for 1 hr at 37°C on LTA coated plates (Ref.2,3,8). Immunofluorescence (Ref.4,9): 60' in PBS/0.02%BSA/0.02% Saponin (Ref.4). Western blot (Ref 5-7): A reduced or native sample treatment and run on 15% SDS-Page. Blot was incubated o/n at 4°C with a 1/1000 dilution. The band size is ~17 kDa (Ref.6,7). The typical starting working dilution is 1/50. Positive Control: Culture medium of Gram-positive bacteria. Negative Control: Culture medium of eukaryotic cells.
Reactivity	Streptococcus sobrims
Host	Mouse
Clonality	Monoclonal
Immunogen	Microbial mixture of Streptococcus sobrims HG961, HG962, HG970, and HG977 (Ref.1).
Specificity	The monoclonal antibody 55 recognizes Lipoteichoic Acid (LTA).
Formulation	State: Low Endotoxin State: Liquid Culture Medium with a Low Endotoxin level Preservative: 0.02% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Background	LTA, a glycerol phosphate surface polymer, is a component of the envelope of Gram-positive bacteria. LTA is anchored via its glycolipids to the membrane and carries a polysaccharide chain extending into the peptidoglycan layer of the cell wall. LTA is released spontaneously into the culture medium during growth of gram-positive bacteria. LTA functions as an immune activator with characteristics very similar to lipopolysaccharide (LPS) from Gram-negative bacteria. LTA binds to CD14 and triggers activation predominantly via Toll-like receptor 2. Although LTA is internalized and traffics to the Golgi, the cellular activation in response to LTA occurs at the cell surface.
Reference Data

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