IP10 (CXCL10) Mouse Monoclonal Antibody [Clone ID: 6D4]
CAT#: AM26287FC-N
IP10 (CXCL10) mouse monoclonal antibody, clone 6D4, FITC
Conjugation: Unconjugated Biotin
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CNY 4,909.00
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Specifications
Product Data | |
Clone Name | 6D4 |
Applications | ELISA, IF, IHC, WB |
Recommend Dilution | Immunohistochemistry on frozen sections (4): Tissue sections were fixed 10 minutes in acetone and pretreated with avidin/biotin blocking kit. As positive control chronically inflamed liver was used and as negative control normal liver and isotype matched antibody (Ref.4). The typical starting working dilution is 1:50. Flow cytometry (3): Antibody 6D4 stains human CXCL10. For intracellular staining activated plasmacytoid dendritic cells were fixed with 2% paraformaldehyde and permeabilized with 0.5% saponin. All steps were performed in presence of 0.5 M EDTA to avoid cell aggregation (Ref.3). The typical starting working dilution is 1:50. Immunoassays (1,2). Immunoflourescence (4). Western blot (4): Tissue was homogenized in loading sample buffer and subjected to SDS-PAGE. The band size is ~9 kDa (Ref.4). The typical starting working dilution is 1:50. Positive control: Acitvated plasmacytoid dendritic cells. Negative control: Normal liver. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Recombinant human IP-10 |
Specificity | The monoclonal antibody 6D4 recognizes human C-X-C motif chemokine 10 (IP-10), a protein of 98 amino acids. |
Formulation | PBS Label: FITC State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide |
Concentration | lot specific |
Purification | Protein G |
Conjugation | FITC |
Storage Condition | Store at 2 - 8 °C. |
Gene Name | C-X-C motif chemokine ligand 10 |
Database Link | |
Background | IP-10, also known as CXCL10, functions as ligand for the CXCR3 receptor. IP-10 belongs to the α-chemokine (C-X-C) family, which can be divided in two subfamilies: (1) potent chemoattractants for neutrophils, like IL-8 and (2) potent chemoattractants for lymphocytes, like the IFN gamma inducible protein (IP)-10. IP-10 is produced by a wide variety of cell types ranging from neutrophils, dendritic cells and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV, encephalitis, cutaneous T cell lymphoma, chronic hepatitis, psoriasis and acute anterior uveitis. Various observations strongly suggest a role for the C-X-C chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis. Furthermore IP-10 is associated with acute rejection processes estimated by the predictive properties of urinary IP-10 expression for the short- and long-term graft function after kidney transplantation. |
Synonyms | C-X-C motif chemokine 10, Small-inducible cytokine B10, Gamma-IP10, INP10, SCYB10 |
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