CD61 (ITGB3) (+Beta-3 Integrin) Mouse Monoclonal Antibody [Clone ID: BV3]

Specifications

Product Data
Clone Name	BV3
Applications	ELISA, FC, IF, IHC, IP
Recommend Dilution	Flow Cytometry: Stains the extracellular domain of integrin αvβ3 . The cells were fixed in 4% paraformaldehyde before before analysis . Negative control the primary antibody was omitted (Ref.2). The typical starting working dilution is 1/50. Immunoassays. Immunoflourescence (Ref.3). Immunoprecipitation. Immunohistochemistry on Paraffin Sections: Tissue sections fixed in Histochoice and blocked with 5% BSA (Ref.1). The typical starting working dilution is 1/50. Positive Control: HUVEC cells. Does not work in Western blot.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	The monoclonal antibody BV3 recognizes Human alpha-V/beta-3 integrin present on Human cells.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	integrin subunit beta 3
Database Link	Entrez Gene 3690 Human UniProt ID P05106
Background	Integrins are a superfamily of αβ heterodimeric cell-surface adhesion receptors found in many species. They are expressed on a variety of cells and mediate numerous physiological processes, including inflammation, migration, adhesion and proliferation. The β3 family consist of 2 members: αIIbβ3 and αvβ3, which mediate cell-cell and cell-ECM interactions and are important for cellular migration, regulation of gene expression, cell survival, adhesion and differentiation. All processes which are involved in tissue development, angiogenesis and thrombosis. Each subunit consist of an extracellular domain, a single transmembrane segment and a cytoplasmic tail. They connect to the actin cytoskeleton via adaptor proteins that bind theircytoplasmic tails. Cell matrix adhesions also act as signaling units by their capacity to organize the actin cytoskeleton and to accumulate various signaling intermediates. Integrin αvβ3 was originally identified as the vitronectin receptor. Nevertheless, other ligands include fibrinogen, fibronectin, laminin, thrombospondin, Von Willebrand factor, tenascin, osteopontin and several forms of collagen. The interactions of integrin αvβ3 to those ligands is mediated by the RGD (Arg-Gly-Asp) sequence motif present in these proteins. Deregulation of β3 integrins is involved in e.g. autoimmune diseases, cardiovascular disorders, transplant rejection and tumorigenesis. In contribution to the latter, integrin αvβ3 contribute by supporting growth of small (tumor) blood vessels thereby potentiating the metastatic potential. Overexpression of integrin αvβ3 has been demonstrated in various tumors and activated endothelium.
Synonyms	Integrin beta-3, GP3A, GPIIIa
Reference Data

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