IL2 Receptor alpha (IL2RA) Mouse Monoclonal Antibody [Clone ID: 143-13]

Specifications

Product Data
Clone Name	143-13
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on frozen sections: 10mg/ml (1:40). Suggested positive control: Human tonsil. Has been described to work in FACS.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Stimulated human leukocytes
Specificity	This antibody detects CD25, a 55kD glycoprotein. It recognizes epitope region C of the IL-2 receptor complex.
Formulation	Phosphate buffered saline pH 7.2 Label: Biotin State: Lyophilized Ig fraction Stabilizer: 5 mg/ml bovine serum albumin (BSA) Preservative: 0.05% (v/v) Kathon CG
Reconstitution Method	Reconstitute by adding 0.5ml distilled water.
Concentration	0.4 mg/ml (reconstituted)
Purification	Affinity purified
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	interleukin 2 receptor subunit alpha
Database Link	Entrez Gene 3559 Human UniProt ID P01589
Background	IL-2R (T-cell growth factor receptor) consists of a chain of 55kDa (also known as Tac antigen or p55 or α chain) and a p75 or β chain. IL-2Rα is over-expressed on the surface of activated Tcells, HTLV-1 or -2 positive T-cell lines, and moderately expressed on activated B-cells, NK-cells, and macrophages. CD25 molecule alone appears to function as a low affinity receptor and associates wtih CD122 (IL-2R β chain, p75) and CD132 (common γ chain) to form the high affinity IL-2 receptor complex.
Synonyms	Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen
Note	Antigen distribution: Isolated cells: The antibody stains less than 5% of resting human peripheral blood mononuclear cells in flow cytometry. Tissue sections: Scattered large and small lymphocytes in germinal centers, in follicle mantle, and T-zone.
Reference Data

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