Ebi3 Rat Monoclonal Antibody [Clone ID: DNT27]

Specifications

Product Data
Clone Name	DNT27
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry on Paraffin Sections: 10 µg/ml. Western Blot.
Reactivity	Human, Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	EBI3 / IL-27B antibody was raised against mouse EBI3
Specificity	The monoclonal antibody DNT27 reacts with Mouse Epstein-Barr Virus-induced gene 3 (EBI3).
Formulation	Aqueous buffer containing 0.09% Sodium Azide and stabilizer if necessary State: Aff - Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	Epstein-Barr virus induced gene 3
Database Link	Entrez Gene 10148 HumanEntrez Gene 50498 Mouse UniProt ID O35228
Background	EBI3 is a 229 amino acid cytokine belonging to the type I cytokine receptor family (type 3 subfamily) with a signal peptide, 2 potential N-linked glycosylation sites, an LSDWS motif, 2 fibronectin type-III domains. It is usually induced due to infection of B lymphocytes by Epstein-Barr virus and due to expression of an EBV encoded latent membrane protein. EBI3 is a subunit of two heterodimeric cytokines interleukin-27 (IL27) and IL35. It is a secreted glycoprotein associated with calnexin and is a heterodimer with an alpha and a beta subunit. It is also known to interact with SQSTM1 and is responsible for the rapid clonal expansion of naive but not memory CD4 (+) T-cells. It strongly synergizes with IL-12 and trigger IFN-gamma production of naive CD4 (+) T-cells. It mediates its biologic effects through the cytokine receptor WSX-1/TCCR. It triggers signaling in T cells, B cells, and myeloid cells and along with p35 it acts as an important immunomodulator. It is usually expressed in various hemopoietic tissues and cell lines.
Synonyms	IL-27 subunit beta, IL-27B
Reference Data

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