PF4 Mouse Monoclonal Antibody [Clone ID: RTO]

Specifications

Product Data
Clone Name	RTO
Applications	ELISA, WB
Recommend Dilution	ELISA: Use at 0.016 μg/ml. Western blot.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human Factor IV purified from the supernatant of Thrombin activated platelets.
Specificity	This antibody is specific for Human Platelet Factor IV. AM31850BT-N (clone RTO) is seen to bind free PF4 in the absence of any bound Heparin. This is unlike AM31851BT-N (clone KKO), which binds PF4/heparin complexes to a similar degree as auto-antibodies found in individuals with Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT).
Formulation	PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. Label: Biotin State: Liquid purified IgG fraction.
Concentration	lot specific
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	platelet factor 4
Database Link	Entrez Gene 5196 Human UniProt ID P02776
Background	Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity.
Synonyms	PF-4, Iroplact, Oncostatin-A, CXCL4, SCYB4
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Chemokine signaling pathway, Cytokine-cytokine receptor interaction

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