MHC Class II (I-Ab,d) Mouse Monoclonal Antibody [Clone ID: 25-9-17S]
CAT#: AM31877FC-N
MHC Class II (I-Ab,d) mouse monoclonal antibody, clone 25-9-17S, FITC
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CNY 3,710.00
货期*
5周
规格
Specifications
Product Data | |
Clone Name | 25-9-17S |
Applications | FC |
Recommend Dilution | Flow Cytometry (See Protocols). |
Reactivity | Mouse |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | C3H.SW splenocytes. Donor: C3H lymphoid cells. Fusion Partner: Sp2/0-Ag14 |
Specificity | This Monoclonal Antibody reacts with I-Ab and I-Ad antigens. Cross reaction with H-2p and H-2q was also found. |
Formulation | PBS containing 0.02% Sodium Azideas preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml Label: FITC State: Liquid purified Ig fraction Label: Fluorescein |
Concentration | lot specific |
Purification | Protein G Chromatography |
Conjugation | FITC |
Storage Condition | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. |
Note | Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 106 cells, representing 1 test). 4. To each tube, add 1.0-0.5 μg* of AM31877FC-N per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 μl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: C57BL/6 Cell Concentration : 1x10e6 cells per test. Antibody Concentration Used: 0.5 μg/106 cells. Isotypic Control: FITC Mouse IgG2a Cell Source Percentage of cells stained above control: Thymus: 12.1% Spleen: 52.0% Lymph Node: 35.2% Strain Distribution by Flow Cytometry Analysis: Antibody Concentration: 0.5 µg/106 cells. Strains Tested: See Figure 2. |
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