CD11a / ITGAL Rat Monoclonal Antibody [Clone ID: IBL-6/2]

Specifications

Product Data
Clone Name	IBL-6/2
Applications	FC
Recommend Dilution	This clone has been reported to work in immunohistochemistry (acetone-fixed frozen sections), flow cytometry and immunoprecipitation. The FITC conjugated format is especially useful for direct flow cytometry.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	EL4 (mouse thymoma cell line) Donor: Wistar spleen Fusion Partner: Sp-2/0Ag14
Specificity	Anti-mouse CD11a monoclonal antibody (Clone: IBL-6/2) recognizes the LFA-1 (CD11a) antigen in mice. The LFA-1 is expressed on leukocytes and it mediates cell-cell and cell-adhesion by binding to CD54 and ICAM-2).
Formulation	PBS containing 0.02% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. Label: FITC State: Liquid purified Ig fraction Label: Fluorescein isothiocyanate isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P24063
Synonyms	Integrin alpha-L, LFA1, LFA-1
Note	Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0.5 – 0.2 μg of this antibody per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 μl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: BALB/c Cell Concentration: 1x10e6 cells per test Antibody Concentration Used: 0.2 μg/10e6 cells Isotypic Control: FITC Rat IgG1
Reference Data

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