CD45 / LCA Rat Monoclonal Antibody [Clone ID: IBL-5/25]

CAT#: AM31886FC-L

CD45 / LCA rat monoclonal antibody, clone IBL-5/25, FITC

Conjugation: Biotin FITC PE



Need it in bulk or conjugated?
Get a free quote

CNY 6,853.00


货期*
5周

规格
    • 300 ug

Product images

Specifications

Product Data
Clone Name IBL-5/25
Applications FC
Recommend Dilution Immunohistochemistry on aceton-fixed frozen sections.
Immunoprecipitation.
Flow Cytometry.
Reactivity Mouse
Host Rat
Clonality Monoclonal
Immunogen IL-3 dependent mast cells derived from WB- +/+ mice
Donor: Wistar spleen
Fusion Partner: X63.653. Ag8
Specificity Anti-mouse CD45 monoclonal antibody detects CD45 (L-CA) which is a transmembrane phosphotyrosine phosphatase expressed on leukocytes. This mAb induces the in vitro clustering of mouse lymphocytes (both T and B cells).
Formulation PBS containing 0.02% sodium azide (NaN3) as preservative nd EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Label: FITC
State: Liquid purified Ig fraction
Label: Fluorescein isothiocyanate isomer 1
Concentration lot specific
Conjugation FITC
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name protein tyrosine phosphatase, receptor type, C
Synonyms PTPRC, Leukocyte common antigen, L-CA, T200
Note Protocol: FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add 2.0 – 0.5 μg of this antibody per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
(It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 μl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).
Results:
Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: BALB/c
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 2.0μg/10e6 cells
Isotypic Control: FITC Rat IgG1
Percentage of cells stained above control:
Mesenteric Lymph Node Cells 95%
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...