Fibrinogen (citrullinated) Mouse Monoclonal Antibody [Clone ID: 23H2]

Specifications

Product Data
Clone Name	23H2
Applications	ELISA, FC, IHC
Recommend Dilution	ELISA.  Immunohistochemistry (Reported).
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Deiminated Murine Fibrinogen peptide.
Specificity	Specificity has been tested in ELISA (Figure 1). Cross-react with deiminated Human Fibrinogen. Additional tests for cross reactivity have not yet been performed.
Formulation	PBS, pH 7.2 State: Purified State: Lyophilized purified Ig fraction
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Add 0.05% Sodium Azide for longer storage. Avoid repeated freezing and thawing.
Background	Fibrinogen is a protein produced by the liver which helps stop bleeding by helping blood clots to form. Fibrinogen gets deiminated (conversion from arginin to citrullin) by Peptidyl Arginine Deïminase (PAD) in inflamed joints in patients that develop rheumatoid arthritis.Citrulline, while being an amino acid, is not built into proteins during protein synthesis, as it is not coded for by DNA, yet several proteins are known to contain citrulline. These citrulline residues are generated by a family of enzymes called peptidylarginine deiminases (PADs), which convert arginine into citrulline in a process called citrullination or deimination. Proteins that normally contain citrulline residues include myelin basic protein (MBP), filaggrin, and several histone proteins, while other proteins, like fibrin and vimentin can get citrullinated during cell death and tissue inflammation. Patients with rheumatoid arthritis often (at least 80% of them) develop an immune response against proteins containing citrulline. Although the origin of this immune response is not known, detection of antibodies reactive with citrulline containing proteins or peptides is now becoming an important help in the diagnosis of rheumatoid arthritis.
Synonyms	FGA, FGB, FGG
Reference Data

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