RPP20 (POP7) Mouse Monoclonal Antibody [Clone ID: 1F11]

Specifications

Product Data
Clone Name	1F11
Applications	ELISA, WB
Recommend Dilution	ELISA. Immunoblotting.  Recommended Solvent: 100 mM PBS or Tris-HCl, pH 7.0
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant GST-hRPP20 (NCBI accession number AAC24113, expression vector pGEC-2T), expressed in E.coli.
Specificity	Specificity has been tested in ELISA. In immunoblotting, under less stringent washing conditions, a weaker band of approximately 60 kDa is also detected (Figure 1, Right Panel). Additional tests for cross reactivity have not yet been performed.
Formulation	10 mM Ammonium Bicarbonate buffer State: Purified State: Lyophilized purified Ig fraction Stabilizer: 2 mg BSA
Reconstitution Method	Dissolve in 200 μl buffer for a 0.5 mg/ml antibody concentration in 1% BSA
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C or Add up to 0.05% Sodium Azide for long term storage.
Predicted Protein Size	20 kDa
Gene Name	POP7 homolog, ribonuclease P/MRP subunit
Database Link	Entrez Gene 10248 Human UniProt ID O75817
Background	The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli. Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle-specific proteins. On average, snoRNPs contain about 6-10 protein subunits.
Synonyms	hPOP7, RPP20
Reference Data

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