MRP5 (ABCC5) Rat Monoclonal Antibody [Clone ID: M5II-54]

Specifications

Product Data
Clone Name	M5II-54
Applications	IF, IHC, WB
Recommend Dilution	Western blotting: Use 1/20-1/50 dilution and anti Rat -HRP Immunocytochemistry: Use 1/20-1/50 on acetone fixed cytospin preparations. Immunohistochemistry on Acetone Fixed Frozen Sections: 1/20 followed by incubation with Rabbit anti-Rat IgG (1/25) and a monoclonal Rat APAAP complex (1/50). Alternatively, after incubation with M5II-54 (1/20) and washing, slides can be incubated with biotinylated Rabbit anti-Rat IgG (1/100) and streptavidin conjugated to Horseradish Peroxidase (1/500).
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	Bacterial fusion protein of MRP5, containing amino acids 722-910 of the protein.
Specificity	This Monoclonal antibody M5II-54 reacts with an internal epitope of MRP5, a 190-200 kD transmembrane protein that is closely related to the multidrug resistance protein MRP. It does not cross-react with the Human MDR1, MRP1, MRP2 or MRP3 gene products.
Formulation	State: Supernatant State: Serum Free Culture Supernatant Stabilizer: 0.7% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ATP binding cassette subfamily C member 5
Database Link	Entrez Gene 10057 Human UniProt ID O15440
Background	MRP5 (190-200 kDa) is closely related to MRP4, both lacking the first five membrane spanning regions. MRP5 is a GS-X multi specific organic anion pump (nucleotide analogs). MRP5 may transport DNP-GS and may be inhibited by certain inhibitors of organic anion transport (sulfinpyrazone). MRP5 may also transport organic anions with the anionic moiety of phosphate/phosphonate group, a function which provides the ability to resist against anti cancer drugs 6-MP and thioguanine as well as the anti-HIV drug PMEA.
Synonyms	Multidrug resistance-associated protein 5, pABC11, SMRP, MOAT-C
Note	Mab producing cells: The hybridoma cell line was obtained by fusion of lymph node cells from an immunized rat (Wistar) with SP2/O mouse myeloma cells. Culture Medium: IMDM (Gibco, Paisley, Scotland UK), supplemented with Nutridoma-NS/SP (Boehringer, Indianapolis, USA), without serum or added enzymes. Antibody containing supernatant has been concentrated and filtered through a 0.22 micron filter.
Reference Data

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