Fibronectin (FN1) (cFn) Mouse Monoclonal Antibody [Clone ID: DH1]

Specifications

Product Data
Clone Name	DH1
Applications	ELISA, IHC, WB
Recommend Dilution	Western blotting. Immunohistochemistry on Frozen Sections. Other Immunoassays for cellular and tumor biology.
Reactivity	Chicken, Guinea Pig, Human, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Fibronectin from A8387 fibrosarcoma cells (ref. 21). Hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
Specificity	The antibody is specific to extradomain A (EDA) sequence of a Cellular Fibronectin and recognizes thus only the Cellular Fibronectin (cFN). Clone DHI has been shown to specifically block Chondrocyte condensation in Chicken embryos (ref. 5).
Formulation	PBS State: Ig Fraction State: Liquid Ig fraction Stabilizer: 1.0% (w/v) BSA Preservative: 0.09% (w/v) Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	fibronectin 1
Database Link	Entrez Gene 2335 Human UniProt ID P02751
Background	Fibronectins are diverse high molecular weight cell adhesion glycoproteins found in basement membranes, in the interstitial connective tissue matrix, and in soluble in plasma and other body fluids. In adult tissues most fibronectin is produced by hepatocytes (pFn) that differ from locally produced cellular fibronectins. A monoclonal antibody (AM32091PU-N Clone BF12) has been produced to fibronectin that reacts with both the cellular and plasma forms of the protein and recognizes the cell-binding region in fibronectin. The monoclonal antibody DH1 recognizes only cellular form of Fibronectin. DH1 is specific to the extra domain A (EDA) of the molecule. cFns are found in some basement membranes during development and only in distinct tissues in adult, for instance in smooth muscle and alveolar walls of airways. cFns are abundant in the plasma of carcinoma patients and in the stroma of various carcinomas.
Synonyms	FN1, Cold-insoluble globulin, CIG
Reference Data

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