Mucin-5AC Mouse Monoclonal Antibody [Clone ID: 58M1]

Specifications

Product Data
Clone Name	58M1
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry on Cultured Cells: Strongly positive on ethanol fixed epithelial cells.  Immunochemistry on Frozen Ethanol Fixed Sections. Immunochemistry on Paraffin Sections: Very strong positive reaction with ethanol fixed tissues.  Protease pretreatment is recommended for formalin post-fixed tissues.  This Monoclonal Antibody Clone 58M1 cross-reacts with mucin of different animal species after periodate pretreatment of deparaffinized sections (Mouse, Cow, Rabbit, Pig). Immunoblotting: Strongly positive without beta-mercaptoethanol pretreatment of Mucin solution.
Reactivity	Bovine, Canine, Feline, Monkey, Mouse, Porcine, Rabbit
Host	Mouse
Clonality	Monoclonal
Immunogen	A BALB/c mouse was immunized with mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification). Splenocytes were fused with mouse myeloma SP2/0 cells.
Specificity	This antibody Clone 58M1 reacts with peptide core of Gastric Mucin (MUC5AC).
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.  Avoid repeated freezing and thawing.
Gene Name	mucin 5AC, oligomeric mucus/gel-forming
Database Link	UniProt ID P98088
Background	Mucins are high molecular weight glycoproteins that are found especially in the secretions of mucus membranes. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.
Synonyms	MUC5AC, MUC5, TBM, LeB
Reference Data

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