Mucin-5AC Mouse Monoclonal Antibody [Clone ID: 1-13M1]

Specifications

Product Data
Clone Name	1-13M1
Applications	ELISA, IHC, R, WB
Recommend Dilution	Immunohistochemistry on cultured cells. Immunochemistry on Frozen Ethanol Fixed Sections. Immunochemistry on Paraffin Sections: a protease pretreatment is recommended. This antibody clone 1-13M1 cross reacts with Mucin of different animal species after periodate pretreatment. Immunoblotting: Strongly positive without mercaptoethanol pretreatment of mucin solution. This antibody clone 1-13M1 recognizes Mucin 5AC under non-reducing conditions. ELISA or IRMA in combination with the Clone 9-13M1 or the mixture of anti-M1 monoclonal antibodies.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A BALB/c mouse was immunized with Mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classisfication). Splenocytes were fused with mouse myeloma SP2/0 cells.
Specificity	This antibody clone 1-13M1 is specific for the peptide core of Gastric Mucin (MUC5AC), and more specifically with the 'a' epitope, which is the most abundant amongst the a,b,c,d,e,f, and h protein core epitopes defined by Bara for M1. This antibody has not been cross-adsorbed and may react with related peptides from cat, cow, chicken, monkey, mouse, rat and rabbit. The antibody is negative with hedgehog and pig.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	mucin 5AC, oligomeric mucus/gel-forming
Database Link	Entrez Gene 4586 Human UniProt ID P98088
Background	Mucins are high molecular weight glycoproteins that are found especially in the secretions of mucus membranes. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.
Synonyms	MUC5AC, MUC5, TBM, LeB
Reference Data

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