Cytokeratin 8 (KRT8) (+ KRT18) Mouse Monoclonal Antibody [Clone ID: B22.1/B23.1]

Specifications

Product Data
Clone Name	B22.1/B23.1
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen and Paraffin Sections: 1/25-1/100.  Preparation and Pretreatment:  1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.  2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.  3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step. Positive control: Pancreas, Prostate, Salivary Gland.  Staining pattern: Cytoplasmic.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognizes Cytokeratin 8 and 18. This antibody is used when attempting to demonstrate the presence of Paget cells; there is very little keratin 18 in the normal epidermis so this will only stain Paget cells. This approach facilitates the interpretation using immunostains and is more sensitive than mucin histochemistry.
Formulation	PBS, pH 7.4 State: Supernatant State: Liquid Tissue Culture Supernatant Stabilizer: 0.9% BSA Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	keratin 8
Database Link	Entrez Gene 3856 Human UniProt ID P05787
Background	Cytokeratins 8 / 18 can be found in most simple epithelium, e.g. thyroid, female breast, gastrointestinal tract, and respiratory tract. Adenocarcinomas and most non-keratinizing squamous carcinomas will stain, but keratinizing squamous carcinomas will not.
Synonyms	KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A
Reference Data

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