CD15 Mouse Monoclonal Antibody [Clone ID: BRA4F1]

Specifications

Product Data
Clone Name	BRA4F1
Applications	ELISA, FC, IHC
Recommend Dilution	ELISA. Flow Cytometry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A BALB/c mouse was immunized with K562 (an erythroid-myeloid leukemia cell line). Splenocytes were fused with mouse myeloma SP2.0-Ag14 cells.
Specificity	This antibody specifically detects CD15. CD15 contains the pentasaccharide lacto-N-fucopentatose III and is particularly expressed on granulocytes and mature neutrophils and on a wide variety of tumor cells including myeloid leukemia, breast, colorectal, and lung cancer cells. The mAb BRA4F1 was clustered at the IV th International Workshop on Leucocyte Differentiation Antigens.
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Background	CD15 is a carbohydrate that is referred to as the Lewis X antigen, also known as alpha-(1,3)-fucosyltransferase 4, which is expressed on a variety of glycolipids and glycoproteins  found on the cell surface of  neutrophils, monocytes, eosinophils and non-hematopoietic tissues including embryonic tissues. CD15 is reported to function as an adhesion molecule and is involved in chemotaxis and phagocytosis. Expression of CD15 is also associated with a wide variety of tumour cells including some adenocarcinomas and certain types of myeloid and lymphoid leukaemias.  Reed-Sternberg cells, found in cases of Hodgkins Lymphoma, also express CD15 and antibodies recognising the CD15 antigen are often used to help differentiate between Hodgkins and non-Hodgkins lymphoma.
Synonyms	Lewis X, X-Hapten, Lacto-N-Fucopentaose III, Stage-Specific Embryonic Antigen, SSEA1
Reference Data

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