CD34 Mouse Monoclonal Antibody [Clone ID: EQ-8D11-C1]

Specifications

Product Data
Clone Name	EQ-8D11-C1
Applications	ELISA, FC, IF, WB
Recommend Dilution	Flow Cytometry: 1.2 μg/106 cells.  Immunofluorescence: 1 μg/106 cells.   Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
Reactivity	Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	Genetic immunisation with cDNA encoding the extracellular domain of Ovine CD34
Specificity	This antibody recognizes Ovine CD34. Based on recognition of the complete native protein expressed on transfected mammalian cells. Other species not tested. Cellular Localization: Membrane.
Formulation	PBS, pH 7.2 State: Purified State: Liquid purified Ig fraction Preservative: None
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	CD34 is a type I membrane protein with an apparent MW of approximately 115 kDa. It is a putative adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. CD34 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells (2). It is thought to act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components.
Synonyms	Hematopoietic progenitor cell marker
Reference Data

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