IL3 Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, Neutralize, WB
Recommend Dilution	Sandwich ELISA: In a Sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant Human IL-3 when used with Biotinylated Antigen Affinity Purified Anti-Human IL-3 (Cat.-No PP1025B) as the detection antibody at a concentration of approximately 0.50-1.0 μg/ml. Western Blot: To detect Human IL-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-3 is 0.50-1.0 ng/lane, under reducing conditions and 2.0-4.0 ng/lane, under non-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-3 (1.0 ng/ml), a concentration of 0.007-0.010 μg/ml of this antibody is required.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly purified E.coli derived Recombinant Human IL-3 (Cat.-No PA076)
Specificity	This antibody recognizes Human Interleukin-3 / IL3.
Formulation	PBS without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	interleukin 3
Database Link	Entrez Gene 3562 Human UniProt ID P08700
Background	IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine.
Synonyms	IL-3, Multipotential colony-stimulating factor, Hematopoietic growth factor, P-cell-stimulating factor, MCGF
Reference Data

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