MelanA (MLANA) Mouse Monoclonal Antibody [Clone ID: M2-7C10]

Specifications

Product Data
Clone Name	M2-7C10
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use Antibody without BSA for Coating. Western Blot: 0.5-1 µg/ml. Immunoprecipitaion: 1-2 µg/500 µg protein lysate. Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 1-2 µg/ml. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive Control: SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human MART-1 protein.
Specificity	This MART-1 antibody clone M2-7C10 recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. This MART-1 antibody clone M2-7C10 does not react with Mouse or Rat. Others species not tested. Researchers should use the MART-1 Antibody clone M2-9E3 (Cat.-No AM32813PU), which also recognizes Human MART-1, to detect Mouse or Rat MART-1. The clone M2-7C10 MART-1 antibody labels melanomas and other tumors showing melanocyte differentiation, and is widely used for assessing melanomas (Campoli, 2012, Ohsie, 2012, Collins, 2012). Analysis of melanoma lesions with this antibody shows that there is significant heterogeneity of expression of MART-1 both as a percentage of cells and in intensity of expression (Marincola, 1996). The reactivity of the MART-1 antibody is not restricted to melanoma, and the antibody has also been shown to label some mesenchymal tumors and sarcomas (Campoli, 2012). The exact eptiope recognized by this MART-1 antibody has not been mapped. However, the MART-1 epitope recognized by this antibody appears to be different from that recognized by the MART-1 antibody clone M2-9E3 (Cat.-No AM32813PU) (Kawakami, 1997). Researchers often use more than one antibody against a given specificity to help follow up and validate results. Hence, it may be useful to use both the Cat.-No AM32812PU and Cat.-No AM32813PU antibodies in parallel to obtain additional information about MART-1 expression. Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A/G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	20-22 kDa (Doublet)
Gene Name	melan-A
Database Link	Entrez Gene 2315 Human UniProt ID Q16655
Background	MART-1 (Melanoma Antigen Recognize by T cells 1), also known as Melan-A, is an 18 kDa melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. MART-1 is expressed in melanosomes and the endoplasmic reticulum. MART-1 is the most widely used marker for identifying malignant melanoma, the most deadly form of skin cancer, and facilitating complete removal of the primary tumor (Campoli, 2012). In this regard, MART-1 is used both as a confirmatory marker for melanocyte differentiation in S100 (protein present in melanocytes) positive lesions and a primary marker to evaluate the extent of melanocyte tumors (Ohsie, 2012, Collins, 2012). MART-1 specific monoclonal antibodies have high sensitivity (75-92%) and specificity (95-100%) for melanoma (Campoli, 2012, Oshie et.al, 2012).
Synonyms	Melan-A protein, MLANA, Antigen SK29-AA, LB39-AA, MelanA, MART1
Reference Data

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