S100A1 Mouse Monoclonal Antibody [Clone ID: 4C4.9]

Specifications

Product Data
Clone Name	4C4.9
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use Antibody without BSA for Coating. Western Blot: 0.5-1 µg/ml.  Immunoprecipitation: 1-2 µg/500 µg protein lysate. Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 1-2 µg/ml. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Embedded Sections: Use C4.9 antibody at 0.25-0.5 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.  Recommended Positive Control: Brain, Melanoma.
Reactivity	Bovine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	S100 protein purified from Bovine brain.
Specificity	This Monoclonal antibody stains S100 protein. 4C4.9 stains Schwannomas, ependymomas, astrogliomas; almost all benign and malignant melanomas and their metastases. Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	10-12 kDa
Gene Name	S100 calcium binding protein A1
Database Link	Entrez Gene 6271 Human UniProt ID P23297
Background	S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein. Despite the fact that S-100 protein is an ubiquitous substance, its demonstration is of great value in the identification of several neoplasms, particularly melanomas and their metastases.
Synonyms	S100 Calcium binding protein, S-100 protein, S100A, S100B, S-100 protein alpha chain, S-100 protein beta chain, Astrocyte Marker
Reference Data

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