BCL2 (41-54) Mouse Monoclonal Antibody [Clone ID: 8C8]

Specifications

Product Data
Clone Name	8C8
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: For coating, Use Antibody without BSA. Western blot: 0.5-1 µg/ml.  Immunoprecipitation: 1-2 µg/500 µg protein lysate.  Immunofluorescence: 1-2 µg/ml.  Flow Cytometry: 0.5-1µg/106 cells. Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min. Epitope unmasking in EDTA (compared to Citrate buffer) is superior.  Positive Control: Jurkat, K562, HL-60, or HeLa Cells, Tonsil or follicular lymphomas.
Reactivity	Human, Monkey, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to amino acids 41-54 of Human Bcl-2
Specificity	This antibody recognizes a protein of 25-26kDa, identified as the bcl-2-apha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein.  The Monoclonal 8C8 antibody has been extensively used to monitor the status of Bcl-2 expression in a myriad of normal and cancerous tissue types, developmental stages, as well as in cultured cells.   Cellular Localization: Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.  Negative Species: Mouse and Rat.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	25-26 kDa
Gene Name	B-cell CLL/lymphoma 2
Database Link	Entrez Gene 596 Human UniProt ID P10415
Background	Bcl-2 (Bcl2) is the founding member of the Bcl-2 family. Family members have pivotal roles in regulating apoptosis or death signaling pathways through their control of mitochondrial permeability and cytochrome release (reviewed in Anvekar, 2011; Martinou and Youle, 2011). Bcl-2 derives its name from B cell lymphoma 2 where was first found to be highly expressed in follicular lymphomas with 14;18 reciprocal translocations. There are two isoforms, alpha and beta, generated by alternative splicing and differing in their carboxy termini. Human Bcl-2 alpha is a 239 amino acid (aa) protein and human Bcl-2 beta is a 205 aa protein. Bcl-2 is over expressed in neoplastic germinal centers of a majority of follicular lymphomas, whereas the normal or hyperplastic germinal centers are primarily negative for Bcl-2 expression. Upregulation has also been described in a number of other types of tumors. Bcl-2 expression is often considered to be a marker of cell death status, and over or high expression has often been tied to anti-apoptotic states, or resistance to death. However, the actual status of vulnerability to death can depend on the balance of other Bcl-2 family members present, their interaction with one another, as well as other factors.
Synonyms	BCL2, Bcl-2 alpha
Reference Data

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