CD31 (PECAM1) Mouse Monoclonal Antibody [Clone ID: C31.7]

Specifications

Product Data
Clone Name	C31.7
Applications	ELISA, FC, IF, IHC, IP, WB
Recommend Dilution	ELISA (For coating, Use Antibody without BSA). Western blot: 0.5-1 µg/ml. Immunoprecipitation: 1-2 µg/500 µg protein lysate. Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 0.5-1 µg/ml. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 1-2 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. Positive Control: Jurkat, CCRF-CEM or K-562 Cells. Tonsil, Angiosarcoma.
Reactivity	Human, Monkey, Rabbit
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant CD31 protein.
Specificity	This monoclonal C31.7 antibody recognizes a 100kDa glycoprotein in endothelial cells and 130kDa in platelets. This antibody reacts with endothelial cells in normal tissues and in benign and malignant proliferations. In cryostat sections and blood smears the antibody also stains megakaryocytes, platelets and occasionally plasma cells. It reacts weakly with mantle zone B cells, peripheral T cells, and neutrophils. Antibody to CD31 is of value in the study of benign and malignant vascular tumors. Staining for CD31 has also been used to measure angiogenesis, which reportedly predicts tumor recurrence. Cellular Localization: Cell surface and cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	~100 kDa (endothelium) and ~130 kDa (platelets)
Gene Name	platelet and endothelial cell adhesion molecule 1
Database Link	Entrez Gene 5175 Human UniProt ID P16284
Background	CD31 (PECAM-1, or platelet endothelial cell adhesion molecule-1) is a surface protein expressed by endothelial cells, monocytes, platelets, granulocytes, and lymphocyte subsets, and makes up a large portion of endothelial intercellular junctions. CD31 is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. Reports indicate that CD31 interacts with CD38 and is involved in cellular interactions resulting in wound healing and angiogenesis. Expression of CD31 on CD4+ T lymphocytes, helps to control T lymphocyte activation, because in the absence of CD31, T cells have a greater propensity to become activated, resulting in increased susceptibility to become apoptotic. This impact of CD31 loss becomes most pronounced during severe, inflammatory, and immunological stresses such as those caused by systemic Salmonella infection. This identifies a novel role for CD31 in regulating CD4 T homeostasis.
Synonyms	PECAM-1, EndoCAM, GPIIA'
Reference Data

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