Annexin V (ANXA5) Mouse Monoclonal Antibody [Clone ID: RUU-WAC2A]

Specifications

Product Data
Clone Name	RUU-WAC2A
Applications	FC, IF, IHC
Recommend Dilution	ELISA in combination with a polyclonal antibody raised against Annexin V in Rabbit. Immunofluorescence to parafolmaldehyde fixed cell samples. Immunohistochemistry on Frozen, Immunohistochemistry on Formaldehyde Fixed, Paraffin-Embedded Sections. For Immunohistochemistry of paraffin sections a pretreatment step with 2% amino-propyl-triethoxysilan (in 100% ethanol) and 0,2 % BSA is recommended. The sections are heated for 15 minutes at 100°C in a citrate buffer (10 mM trisodium citrate, pH 6,0). The sections are then further processed by routine procedures. Recommended Dilutions: 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/100-1/500 for immunoblotting applications.
Reactivity	Human, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Annexin A5.
Specificity	RUU-WAC2A is a monoclonal that specifically reacts with Annexin A5. This monoclonal antibody is directed to the phospholipid binding domain of Annexin A5 and as such it inhibits Annexin A5 binding to anionic phospholipid membranes for more than 95%. RUU-WAC2A does not remove Annexin A5 bound to phospholipid surfaces indicating that the epitope is not accessible after interaction with Annexin A5. RUU-WAC2A reacts against human recombinant and endogenous annexin A5. It shows no cross reactivity with Annexins I, II, III, IV, VI, VII and VIII in ELISA or in immunoprecipitation. It does not recognise Monkey and Rat Annexin V in ELISA.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	annexin A5
Database Link	Entrez Gene 308 Human UniProt ID P08758
Background	Annexin A5 (or annexin V) is a cellular protein of the annexin family. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin, and also to inhibit the activity of phospholipase A1. Annexin A5 forms a shield around negatively-charged phospholipid molecules, which blocks the entry of phospholipids into coagulation (clotting) reactions. Antibodies directed against annexin A5 are the cause of the antiphospholipid syndrom in which the formation of the shield is disrupted by antibodies. Without the shield, there is an increased quantity of phospholipid molecules on cell membranes, speeding up coagulation reactions and causing the blood-clotting characteristic of the antiphospholipid antibody syndrome. Annexin A5 is used as a probe in the annexin A5 affinity assay to detect cells that have expressed phosphatidylserine on the cell surface, a feature found in apoptosis aswell as other forms of cell death (1,2). Platelets also expose phosphatidyl-serine on their surface when activated, which serves as binding site for various coagulation factors. Annexin A5 has been shown to interact with kinase insert domain receptor (3) and integrin beta 5 (4).
Synonyms	Annexin-5, Annexin V, Lipocortin V, Endonexin II, Calphobindin I, Anchorin CII, ANX5, ENX2, PP4, CBP-I, PAP-I, VAC-alpha
Reference Data

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