beta Actin (ACTB) Mouse Monoclonal Antibody [Clone ID: 4C2]

Specifications

Product Data
Clone Name	4C2
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA. Flow cytometry. Western blot. Immunocytochemistry. Immunohistochemistry on Frozen sections. Immunohistochemistry on Paraffin-embedded sections. Recommended Dilutions: 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/100-1/500 for immunoblotting applications.
Reactivity	Chicken, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide comprising the N-terminal nonapeptide of β-Cytoplasmic Actin with an acetylated N-terminus coupled to KLH through the cysteine residue
Specificity	This 4C2 Monoclonal antibody is highly specific for β-Cytoplasmic Actin. The antibody does not cross react with other Actin isoforms.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	actin, beta
Database Link	Entrez Gene 60 Human UniProt ID P60709
Background	Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. β- and γ-cytoplasmic actin play crucial roles during various key cellular processes. Whereas β-actin is preferentially localized in stress fibers, circular bundles and at cell-cell contacts, suggesting a role in cell attachment and contraction, γ-actin displays a more versatile organization, according to cell activities. In moving cells, γ-actin is mainly organized as a meshwork in cortical and lamellipodial structures, suggesting a role in cell motility. β- and γ-actin depleted fibroblasts exhibit distinct changes in motility compared with their controls, suggesting a specific role for each isoform in cell locomotion.
Synonyms	Actin cytoplasmic 1, Beta-Actin
Reference Data

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