gamma Actin (ACTG1) (Cytopl.) Mouse Monoclonal Antibody [Clone ID: 2A3]

CAT#: AM33003PU-N

gamma Actin (ACTG1) (Cytopl.) mouse monoclonal antibody, clone 2A3, Purified



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CNY 5,186.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 2A3
Applications ELISA, FC, IF, IHC, WB
Recommend Dilution ELISA. 
Flow Cytometry. 
Immunoblotting

Immunocytochemistry on fixed cells.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on 
Paraffin-Embedded Sections. 
Recommended Dilutions: 1/100-1/500 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/1000-1/5000 for immunoblotting applications.
Reactivity Chicken, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
Host Mouse
Clonality Monoclonal
Immunogen Peptide comprising the N-terminal nonapeptide of γ-cytoplasmic Actin with an acetylated N-terminus coupled to KLH through the cysteine residue.
Specificity This 2A3 Monoclonal antibody is highly specific for γ-cytoplasmic Actin (γ-actin 1; encoded by ACTG1).
It does not cross-react with the γ-actin isoform expressed in the enteric smooth muscle tissues (γ-actin 2; encoded by ACTG2).
Formulation PBS
State: Purified
State: Liquid purified IgG fraction
Preservative: 0.09% Sodium Azide
Concentration lot specific
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles.
Gene Name actin gamma 1
Background Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus,  but the sequence of each of these actins is highly conserved in higher vertebrates.
β- and γ-cytoplasmic actin play crucial roles during various key cellular processes. Whereas β-actin is preferentially localized in stress fibers, circular bundles and at cell-cell contacts, suggesting a role in cell attachment and contraction, γ-actin displays a more versatile organization, according to cell activities. In moving cells, γ-actin is mainly organized as a meshwork in cortical and lamellipodial structures, suggesting a role in cell motility. β- and γ-actin depleted fibroblasts exhibit distinct changes in motility compared with their controls, suggesting a specific role for each isoform in cell locomotion.
Synonyms ACT; ACTB; ACTG; DFNA20; DFNA26; Gamma-actin
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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