gamma Actin (ACTG1) (Cytopl.) Mouse Monoclonal Antibody [Clone ID: 2A3]

Specifications

Product Data
Clone Name	2A3
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA. Flow Cytometry. Immunoblotting Immunocytochemistry on fixed cells. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Sections. Recommended Dilutions: 1/100-1/500 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/1000-1/5000 for immunoblotting applications.
Reactivity	Chicken, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide comprising the N-terminal nonapeptide of γ-cytoplasmic Actin with an acetylated N-terminus coupled to KLH through the cysteine residue.
Specificity	This 2A3 Monoclonal antibody is highly specific for γ-cytoplasmic Actin (γ-actin 1; encoded by ACTG1). It does not cross-react with the γ-actin isoform expressed in the enteric smooth muscle tissues (γ-actin 2; encoded by ACTG2).
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	actin gamma 1
Database Link	Entrez Gene 71 Human UniProt ID P63261
Background	Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. β- and γ-cytoplasmic actin play crucial roles during various key cellular processes. Whereas β-actin is preferentially localized in stress fibers, circular bundles and at cell-cell contacts, suggesting a role in cell attachment and contraction, γ-actin displays a more versatile organization, according to cell activities. In moving cells, γ-actin is mainly organized as a meshwork in cortical and lamellipodial structures, suggesting a role in cell motility. β- and γ-actin depleted fibroblasts exhibit distinct changes in motility compared with their controls, suggesting a specific role for each isoform in cell locomotion.
Synonyms	ACT; ACTB; ACTG; DFNA20; DFNA26; Gamma-actin
Reference Data

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