Epcam Rat Monoclonal Antibody [Clone ID: G8.8]

Specifications

Product Data
Clone Name	G8.8
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Western blotting: 1/100-1/1000. Flow Cytometry: 1/25–1/200. Immunocytochemistry. Immunoprecipitation. Immunohistochemistry on Frozen Sections: 1/25–1/200 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	This antibody is derived by fusion of X63-Ag8.653 mouse myeloma cells with spleen lymphocytes from rats repeatedly immunized with glycoconjugates from BALB/c Mouse derived medullary thymic epithelial cells.
Specificity	The G8.8 antibody reacts with CD326/Ep-CAM (Epithelial Cell Adhesion Molecule), also known as gp-40 in the Mouse. Ep-CAM is a 40-42-kDa cell-surface glycoprotein expressed on thymic epithelial cells, thymic dendritic cells, immature thymocytes, a small subset of peripheral T lymphocytes, intestinal epithelium, kidney-collecting tubule epithelium, keratinocytes, Langerhans cells, as well as lymph node and splenic dendrititc cells.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	epithelial cell adhesion molecule
Database Link	Entrez Gene 17075 Mouse UniProt ID Q99JW5
Background	Ep-CAM is a 40 kD glycoprotein and can be detected at the basolateral membrane of the majority of epithelial tissues, where it is intricately linked with the cadherin-catenin complex and hence the fundamental WNT pathway responsible for intracellular signalling and polarity. This antigen functions as a homotypic calcium-independent cell adhesion molecule. Of particular interest, Ep-CAM appears to be overexpressed by the majority of human epithelial carcinomas, including colorectal, breast, prostate, head and neck, and hepatic carcinomas. The antigen is being used as a target for immunotherapy of human carcinomas. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation.
Synonyms	Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18
Reference Data

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