EPCAM Mouse Monoclonal Antibody [Clone ID: VU-1D9]

Specifications

Product Data
Clone Name	VU-1D9
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for Coating. Western Blot: 0.5-1 µg/ml. Detects a band of approximately 38kDa in HT-29 cell lysates. Immunoprecipitaion: 0.5-1 µg/500 µg protein lysate. Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 1-2 µg/ml. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1 mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C Positive Control: HT29 cells or breast tumor.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Small cell lung carcinoma cells.
Specificity	This antibody is specific for CD326, also known as Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. Cellular Localization: Cell surface. Negative Species: Rat.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A/G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	40-43 kDa
Gene Name	epithelial cell adhesion molecule
Database Link	Entrez Gene 4072 Human UniProt ID P16422
Background	Epithelial Cell Adhesion Molecule (Ep-CAM), a 38kD cell surface antigen otherwise known as CD326, MOC31 and Ber-EP4. CD326 is a type 1 transmembrane glycoprotein. It is expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells.
Synonyms	Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18
Reference Data

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