Epcam Mouse Monoclonal Antibody [Clone ID: SPM535]

Specifications

Product Data
Clone Name	SPM535
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Western blot analysis: 1/100-1/200. Flow Cytometry: 5-10 μl/106 cells. Immunofluorescence: 1/50-1/100. Immunoprecipitation: 5-10 μl/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 1/50-1/100 for 30 minutes at RT. Formalin/paraffin tissues REQUIRE digestion of tissue sections with pepsin at 1 mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C. Positive Control: Rat Small intestine or Colon.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat small intestine cells.
Specificity	Epitope of SPM534 and SPM535 monoclonal antibodies are different. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Cellular Localization: Cell surface.
Formulation	State: Supernatant State: Liquid Bioreactor Concentrate Preservative: 0.05% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	40-43 kDa
Gene Name	epithelial cell adhesion molecule
Database Link	Entrez Gene 171577 Rat UniProt ID O55159
Background	EpCAM, or CD326, is an ~40 kDa transmembrane glycoprotein involved in a number of cellular processes including adhesion, proliferation, maintenance of stemness, migration and invasion (Spizzo, 2012, Denzel, 2012). It is expressed on most, but not all, normal epithelia and their corresponding malignancies. Although the role of EpCAM expression in tumorigenesis remains to be fully elucidated, it may downregulate immunity and help tumors actively escape from immune surveillance. Since the 1980s, EpCAM has been recognized as a pan-carcinoma antigen marker because of its widespread expression on epithelium and their derived tumors (Litnov, 1994; Ruf, 2007). More recently, it has also been identified as a stem cell marker, and it is thought that EpCAM antibody positive tumor cells may have stem-like properties (Flatmark, 2011). EpCAM antibody is widely used to help distinguish epithelial from non-epithelial neoplasms. EpCAM antibody positive tumors are epithelium derived, whereas EpCAM antibody negative tumors can originate from either non-epithelial or epithelial tissues. EpCAM antibody has also been used for identifying circulating tumor cells (Saif, 2012, de Albuquerque, 2012). It is often used as part of a panel with other tumor or tissue antibody markers when classifying tumors of known, suspected or elusive origin. EpCAM expression may be upregulated in tumors and overexpression can most pronounced on tumor initiating cells (Imrich, 2012). Overexpression may be an indication of early malignancy and correlate with a poor prognosis (Ruf, 2007).
Synonyms	Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18
Reference Data

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