Carbonic Anhydrase IX (CA9) (Renal Cell Carcinoma Marker) Mouse Monoclonal Antibody [Clone ID: SPM487]

Specifications

Product Data
Clone Name	SPM487
Applications	FC, IF, IHC, WB
Recommend Dilution	ELISA: Use BSA free antibody for coating. Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 1-2 µg/ml. Western Blot: 0.5-0.1 µg/ml. Immunoprecipitation: 1-2 μg/500 µg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Normal kidney or renal cell carcinoma.
Reactivity	Equine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Microsomal fraction of human renal cortical tissue homogenate
Specificity	Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This Monoclonal antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation. Cellular Localization: Cell Surface and Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	200 kDa
Gene Name	carbonic anhydrase 9
Database Link	Entrez Gene 768 Human UniProt ID Q16790
Background	In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Of other normal tissues examined, the gp200 is also localized along the luminal surfaces of breast lobules and ducts, the luminal surface of the epididymal tubular epithelium, within the cytoplasm of parathyroid parenchymal cells, and focally within the colloid of thyroid follicles. Thirty-one other normal tissues do not express similar or cross-reacting antigens. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas.
Synonyms	Carbonic anhydrase IX, Carbonate dehydratase IX, Membrane antigen MN, P54/58N, pMW1, CA9, CA IX, G250
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.