Thyroglobulin (TG) Mouse Monoclonal Antibody [Clone ID: SPM517]

Specifications

Product Data
Clone Name	SPM517
Applications	FC, IHC, WB
Recommend Dilution	ELISA: Use BSA free Antibody forr coating. Western Blot: 0.5-1 µg/ml. Flow Cytometry: 0.5-1 µg/106 cells. Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Thyroid.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human thyroid follicular cells.
Specificity	This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of Thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-Thyroglobulin, combined with anti-Calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-Thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms. Cellular Localization: Cytoplasmic, Secreted.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	660 kDa (Dimeric Form)
Gene Name	thyroglobulin
Database Link	Entrez Gene 7038 Human UniProt ID P01266
Background	Thyroglobulin is a 660 kDa dimeric preprotein with mutiple glycosylation sites is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformation maturation in the endoplasmic reticulation. Thyroglobulin dimerization as well as transport of thyroglobulin to the Golgi complex is calcium dependent. Thyroglobulin defects resulting from defective dimer formation and export to the Golgi is thought to cause some types of goiter. Antibody against thryoglobulin may be produced by individuals with other diseases arising from the gland such as Hashimoto's or Graves disease. Hence the presence of thyroglobulin autoantibodies can help to identify disease. Antibody to thyroglobulin has been shown to be useful for the identification of papillarly and follicular thyroid carcinoma; thyroglobulin antibody positive lesions are of thyroidal origin. Carcinomas of nonthyroidal origin do not express thyroglobulin and hence are thyroglobulin antibody negative. It is important to note though that not every type of thyroidal lesion is thyroglobulin antibody positive, a number of forms are negative. Hence a negative result does not necessarily rule out that a given lesion or metastasis originated from the thyroid gland.
Synonyms	Thyreoglobulin, TGN
Reference Data

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