TGF alpha (TGFA) Mouse Monoclonal Antibody [Clone ID: 1E8-G6]

CAT#: AM33319PU-S

TGF alpha (TGFA) mouse monoclonal antibody, clone 1E8-G6, Purified

Size: 20 ug 100 ug



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CNY 5,606.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 1E8-G6
Applications FC, IF, IHC, IP, WB
Recommend Dilution ELISA: Use BSA Free Antibody for Coating. 
Western Blotting (Not tested).
Flow Cytometry: 0.5-1 µg/106 cells.
Immunofluorescence: 1-2 µg/ml.
Immunoprecipitation: 1-2 µg/ml.
Immunohistochemistry on Frozen and Formalin-Paraffin Sections:
 1-2 µg/ml for 30 minutes at RT.  
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: Jurkat or Ramos cells, Heart, Kidney, Pituitary, Breast cancer, Melanoma.
Reactivity Human, Rabbit, Zebrafish
Host Mouse
Clonality Monoclonal
Immunogen A 10-amino acid synthetic peptide (aa34-43) from Human TGF alpha
Specificity This Monoclonal Antibody reacts with the TGF alpha and shows no cross- reaction with EGF and the neuropeptide synenkephalin.
The staining with this Monoclonal Antibody is completely blocked by the peptide used for raising this antibody. TGFα (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cel l proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Cellular Localization: Cytoplasmic and Extracellular (Secreted).
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
DO NOT FREEZE!
Predicted Protein Size ~6 kDa
Gene Name transforming growth factor alpha
Synonyms Transforming growth factor alpha, ETGF
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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