Fibronectin (FN1) Mouse Monoclonal Antibody [Clone ID: HFN7.1]

Specifications

Product Data
Clone Name	HFN7.1
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use Antibody without BSA for Coating. Biological Blockade: Use Azide Free Antibody. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: SW156 cells or Kidney.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human Fibronectin purified from serum by Affinity Chromatography on gelatin-sepharose.
Specificity	This antibody recognizes Human Fibronectin. Other species not tested. Fibronectin is a dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This Monoclonal Antibody is directed against the peptide core and reacts with both the plasma and cellular forms of Fibronectin. It blocks the Fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of Fibronectin to its receptor on the cell surface. Cellular Localization: Connective tissue matrix.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	220 kDa (monomer); 440 kDa (dimer)
Gene Name	fibronectin 1
Database Link	Entrez Gene 2335 Human UniProt ID P02751
Background	Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They occur as dimers of two 250 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.
Synonyms	FN1, Cold-insoluble globulin, CIG
Reference Data

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