Myogenin (MYOG) Mouse Monoclonal Antibody [Clone ID: F5D]

Specifications

Product Data
Clone Name	F5D
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: For coating, order Antibody without BSA. Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 0.5-1 µg/ml. Western Blot: 0.5-1 µg/ml.  Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.  Immunohistochemistry on Frozen Sections.  Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.  Positive Control: Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
Reactivity	Canine, Feline, Human, Mouse, Porcine, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat Myogenin peptide (aa 73-94) followed by Rat Myogenin recombinant fragment (aa30-224) Epitope: aa138-158.
Specificity	Anti-Myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms’ tumor.  Cellular Localization: Nuclear.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A/G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	34 kDa
Gene Name	myogenin (myogenic factor 4)
Database Link	Entrez Gene 4656 Human UniProt ID P15173
Background	Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). Family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. Family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle specific genes, family members activate their own transcription and transactivate the transcription of other family members. For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of the endogenous myogenin gene as well as transactivation of MyoD. Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. Each member of the family has distinct roles in muscle development; myogenin plays a key role in muscle maturation. Myogenin migrates at a molecular weight of ~34 kDa by SDS-PAGE.
Synonyms	MYOG, MYF4, Myogenic factor 4, BHLHC3
Reference Data

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