TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: 4C6-H8]

Specifications

Product Data
Clone Name	4C6-H8
Applications	FC, IF, IHC, IP
Recommend Dilution	ELISA: Use free BSA Antibody for coaating. Flow Cytometry: 0.5-1 µg/million cells.  Immunofluorescence: 0.5-1 µg/ml. Immunoprecipitation (Not tested): 1-2 µg/500 µg protein lysate.  Immunohistochemistry on Frozen and Formalin-Fixed Sections: 2-4 µg/ml for 30 minutes at RT.  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.  Positive Control: HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil.
Reactivity	Canine, Feline, Human, Mouse, Rabbit, Rat, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	A hexadecapeptide corresponding to amino acids 115-130 of Human TNF-alpha, conjugated to Thyroglobulin.
Specificity	Recognizes TNF-alpha.  Clone 4C6-H8 reacts with Tumor Necrosis Factor-alpha. It reacts on paraffin sections with macrophages, in which the cytoplasma is stained. Some keratinocytes are also positive (tonsils). Cellular Localization: Cytoplasmic and extracellular (secreted).
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	17 kDa
Gene Name	tumor necrosis factor
Database Link	Entrez Gene 7124 Human UniProt ID P01375
Background	Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Synonyms	TNF, TNF-a, TNFA, TNFSF2, Cachectin
Reference Data

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