Involucrin (IVL) Mouse Monoclonal Antibody [Clone ID: SY5]

CAT#: AM33368PU-S

Involucrin (IVL) mouse monoclonal antibody, clone SY5, Purified

Size: 20 ug 100 ug



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CNY 5,606.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Clone Name SY5
Applications ELISA, FC, IF, IHC, IP, WB
Recommend Dilution ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 1-2 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 1-2 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 1-2 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues is ENHANCED by digestion with trypsin (or Protease XXV) at 1mg/ml PBS for 5 min at 37°C.
Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
Reactivity Canine, Human, Monkey, Porcine, Gorilla
Host Mouse
Clonality Monoclonal
Immunogen Purified Involucrin from Human keratinocytes.
Specificity It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured Human keratinocytes, this MAb reacts with a 120kDa protein.
It stains the Involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in Gorilla and owl Monkey, 66kDa in Dog, and a doublet of 105kDa in Pig.
Its epitope maps between codon 421-568 of Human Involucrin. 
Cellular Localization: Cytoplasmic.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
Predicted Protein Size 66-170 kDa
Gene Name involucrin
Background Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it.
Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Synonyms involucrin
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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