HSP27 (HSPB1) Mouse Monoclonal Antibody [Clone ID: G3.1]

CAT#: AM33370PU-S

HSP27 (HSPB1) mouse monoclonal antibody, clone G3.1, Purified

Size: 20 ug 100 ug



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CNY 5,606.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name G3.1
Applications FC, IF, IHC, IP, WB
Recommend Dilution ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: ~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor.
Reactivity Chimpanzee, Chicken, Human, Monkey, Mouse, Rat, Sheep
Host Mouse
Clonality Monoclonal
Immunogen Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Specificity It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced “p29” and “24K” protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Cellular Localization: Predominantly cytoplasmic with some nuclear.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
Predicted Protein Size 27 kDa
Gene Name heat shock protein family B (small) member 1
Synonyms Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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