HSP27 (HSPB1) Mouse Monoclonal Antibody [Clone ID: G3.1]
CAT#: AM33370PU-S
HSP27 (HSPB1) mouse monoclonal antibody, clone G3.1, Purified
Size: 20 ug
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CNY 5,606.00
货期*
5周
规格
Specifications
Product Data | |
Clone Name | G3.1 |
Applications | FC, IF, IHC, IP, WB |
Recommend Dilution | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: ~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. |
Reactivity | Chimpanzee, Chicken, Human, Monkey, Mouse, Rat, Sheep |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells. |
Specificity | It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced “p29” and “24K” protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells. Cellular Localization: Predominantly cytoplasmic with some nuclear. |
Formulation | 10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide |
Concentration | lot specific |
Purification | Protein A/G Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C. |
Predicted Protein Size | 27 kDa |
Gene Name | heat shock protein family B (small) member 1 |
Database Link | |
Synonyms | Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25 |
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