CD7 Mouse Monoclonal Antibody [Clone ID: B-B7]

Specifications

Product Data
Clone Name	B-B7
Applications	FC, IF, IHC
Recommend Dilution	- Flow cytometry: for analysis of blood and bone marrow samples. Used in flow cytometry to analyze T and NK cell subsets and for the characterization of T-ALL and other T cell lymphoblastic leukemias (see "Protocols" below). - Immunofluorescence / Immunohistochemistry: using cytospots or frozen tissue sections. Suitable for the identification of T cells in tissues and diagnosis of T cell neoplasms.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	Clone B-B7 recognizes a 40 kD T cell and NK cell antigen.
Formulation	0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2% BSA, 0.09% sodium azide State: Aff - Purified State: Liquid purified protein
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	CD7 molecule
Database Link	Entrez Gene 924 Human UniProt ID P09564
Background	CD7 is the earliest antigen marker expressed in the T lineage, being found on T cell precursors in fetal liver and thorax prior to thymic colonization and in thymus and bone marrow. The function of CD7 antigen is unknown and the natural ligand for CD7 has not yet been identified. CD7 monoclonal antibodies co-stimulate T cell proliferation and induce second messengers, while soluble recombinant CD7 has been reported to inhibit antigen-specific proliferation and a mixed lymphocyte reaction. CD7 is a marker for pluripotential stem cell leukemias and T cell acute lymphocytic leukemia (T-ALL). The antigen is frequently lost on large cell T cell lymphomas. The CD7 antigen may also be expressed on myeloblastic leukemias.
Synonyms	GP40, TP41, Leu-9
Note	1. Conjugates with brighter fluorochromes, like PE and APC, will have a greater separation than those with dyes like FITC. When populations overlap, the percentage of positive cells using a selected marker can be affected by the choice of fluorescent label. 2. Use of monoclonal antibodies in patient treatment can interfere with antigen target recognition by this reagent. This should be taken into account when samples are analyzed from patients treated in this fashion. 3. Reagent data performance is based on EDTA-treated blood. Reagent performance can be affected by the use of other anticoagulants. Protocol: Flow cytometry method for use with purified monoclonal antibodies 1. Add 100 µl of EDTA-treated blood (i.e. approx. 10e6 leukocytes) to a 5 ml reagent tube. The content of one tube is sufficient to perform one test. 2. Add to each tube 10 µl of purified monoclonal antibody. (Appropriate mouse Ig isotype control samples should always be included in any labeling study). Vortex the tube to ensure thorough mixing of antibody and cells. 3. Incubate the tube for 15 minutes at room temperature in the dark. 4. Wash the labeled cells by adding 2 ml of PBS containing 0.001% (v/v) Heparin, vortexing and centrifuging (2 min 1000 x g) and discard the supernatant. 5. Add 50 µl of appropriate dilution of F(ab)2 Rabbit Anti Mouse IgG fluorescent conjugate (e.g.FITC or R-PE) in PBS containing 0.001% (v/v) Heparin to the tube. It is recommended that the tube is protected from light. 6. Mix by vortexing and incubate for 15 minutes at room temperature in the dark. 7. Add 100 µl of a lyse reagent and mix immediately. 8. Incubate for 10 minutes at room temperature in the dark. 9. Add 2 ml of demineralized water and incubate for 10 minutes in the dark. 10. Centrifuge the labeled cell suspension for 2 minutes at 1000 x g. 11. Remove the supernatant and resuspend the cells in 200 µl of PBS. 12. Analyze by flow cytometry within four hours (alternatively, the cells may be fixed by 0.05% of formaline in buffered saline for analysis the next day. Some antigens are readily destroyed upon fixation and this should be taken into account when using this alternative).
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Hematopoietic cell lineage

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