BHMT Mouse Monoclonal Antibody [Clone ID: 3D6]

Specifications

Product Data
Clone Name	3D6
Applications	ELISA, WB
Recommend Dilution	ELISA. Western blot: 1/1000-1/2000. Detects a band of approximately 42 kDa in Mouse liver lysates.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human BHMT (amino acids 1-406) purified from E. coli.
Specificity	This antibody detects Betaine Homocysteine Methyltransferase (BHMT), a 45kDa cytosolic enzyme that uses betaine as a methyl donor to catalyze the remethylation of homocysteine to methionine. This remethylation process is essential as it leads to the synthesis of the critical methyl group donor S-adenosylmethionine (SAM), whilst conserving methionine and detoxifying homocysteine. BHMT is expressed in the liver, kidney, and optic lens. Defects in homocysteine metabolism have a number of adverse side effects, however as yet changes in BHMT function has not been directly linked to them.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-G Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	betaine--homocysteine S-methyltransferase
Database Link	Entrez Gene 635 Human UniProt ID Q93088
Background	Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine.
Synonyms	Betaine--homocysteine S-methyltransferase 1
Reference Data
Protein Pathways	Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways

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