ICT1 (MRPL58) (30-206) Mouse Monoclonal Antibody [Clone ID: AT1E9]

Specifications

Product Data
Clone Name	AT1E9
Applications	ELISA, FC, WB
Recommend Dilution	ELISA. Western blot: Recommended starting dilution is 1:1000. Flow cytometry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human ICT1 (30-206aa) purified from E. coli
Specificity	This antibody detects ICT1 at aa30-206.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	immature colon carcinoma transcript 1
Database Link	Entrez Gene 3396 Human UniProt ID Q14197
Background	Peptidyl-tRNA hydrolase ICT1 acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. This neoplastic-induced deviation is marked by a change in expression of several mRNAs. ICT1 is a member of the prokaryotic/mitochondrial release factor family whose expression is downregulated over 4 fold upon colon stem cell differentiation. This downregulation of ICT1 could lead to its use as a marker for detection of colon carcinomas.
Synonyms	Digestion substraction 1, DS-1
Reference Data

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