KRT6A (C-term) Mouse Monoclonal Antibody [Clone ID: SPM269]

Specifications

Product Data
Clone Name	SPM269
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 10-15 min at RT. Positive Control: Suprabasal cells of the outer hair follicle root sheath in skin. Basal cell carcinoma.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from C-terminus of Human keratin 6.
Specificity	This Monoclonal Antibody recognizes a protein of 56kDa, identified as Cytokeratin 6 (CK6). Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	56 kDa
Gene Name	keratin 6A
Database Link	Entrez Gene 3853 Human UniProt ID P02538
Background	In Humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.
Synonyms	Cytokeratin 6, KRT-6, K6, KRT6A, KRT6D, KRT6B, KRT6C, KRT6E, KRTL1
Reference Data

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