PCNA Mouse Monoclonal Antibody [Clone ID: SPM350]

CAT#: AM50151PU-S

PCNA mouse monoclonal antibody, clone SPM350, Purified

Size: 20 ug 100 ug



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CNY 5,606.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name SPM350
Applications FC, IF, IHC, IP, WB
Recommend Dilution ELISA: Use BSA free Antibody for coating.
Flow Cytometry: 0.5-1 μg/million cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blotting: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.25-0.5 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: Tonsil or reactive lymph node.
Reactivity Chicken, Drosophila, Human, Mouse, Rat, Yeast, Zebrafish
Host Mouse
Clonality Monoclonal
Immunogen Rat PCNA/Protein A fusion protein.
Specificity Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.
This MAb is excellent for multiple applications.
Cellular Localization: Predominantly nuclear, some cytoplasmic.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
Predicted Protein Size 36 kDa
Gene Name proliferating cell nuclear antigen
Synonyms Cyclin
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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