alpha smooth muscle Actin (ACTA2) Mouse Monoclonal Antibody [Clone ID: SPM332]

Specifications

Product Data
Clone Name	SPM332
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/ml. Western Blotting: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistology (Formalin-Fixed): 0.5-1.0 μg/ml for 30 minutes at RT. (Staining of Formalin-Fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min.) Positive Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma.
Reactivity	Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Baboon
Host	Mouse
Clonality	Monoclonal
Immunogen	N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
Specificity	Actin is a major component of the cytoskeleton and is present in most cell types. This Monoclonal Antibody is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This  Monoclonal Antibody does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin. Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	42 kDa
Gene Name	actin, alpha 2, smooth muscle, aorta
Database Link	Entrez Gene 59 Human UniProt ID P62736
Synonyms	ACTSA, ACTVS, Alpha-actin-2
Reference Data

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