Sumo 1 (SUMO1) Mouse Monoclonal Antibody [Clone ID: SPM571]

Specifications

Product Data
Clone Name	SPM571
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/million cells.  Immunofluorescence: 0.5-1 µg/ml.  Western Blotting: 0.5-1 µg/ml. Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.  Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 µg/ml for 30 minutes at RT.  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Breast carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human SUMO1 protein.
Specificity	This MAb is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3.  Cellular Localization: Predominantly nuclear with some cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	11.5 kDa (Monomer); 90 kDa (Heteromer)
Gene Name	small ubiquitin-like modifier 1
Database Link	Entrez Gene 7341 Human UniProt ID P63165
Background	The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.
Synonyms	SMT3C, SMT3H3, UBL1, GMP1, SMT3 homolog 3, Sentrin
Reference Data

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