CD20 (MS4A1) Mouse Monoclonal Antibody [Clone ID: B9E9]
CAT#: AM50217PU-S
CD20 (MS4A1) mouse monoclonal antibody, clone B9E9, Purified
Size: 20 ug
Need it in bulk or conjugated?
Get a free quote
CNY 5,606.00
货期*
5周
规格
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Clone Name | B9E9 |
Applications | IF, IHC, IP |
Recommend Dilution | ELISA: Use BSA free Antibody for coating. Functional Studies: (Use Antibody without BSA and Azide. Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 0.5-1 µg/ml. Immunoprecipitation: 0.5-1 μg/500 µg protein lysate. Immunohistochemistry on Frozen Sections: 0.5-1.0 µg/ml for 30 min at RT. Positive Control: Daudi, Raji, and U266, and human lymphocytes. In normal lymph nodes and tonsils, CD20 is predominantly detected on B cells in germinal centers. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Lymphoblastoid cell line Daudi. |
Specificity | Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This Monoclonal Antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. Cellular Localization: Predominantly cell surface with some cytoplasmic. |
Formulation | 10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide |
Concentration | lot specific |
Purification | Protein A/G Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C. |
Predicted Protein Size | 33-37 kDa |
Gene Name | membrane spanning 4-domains A1 |
Database Link | |
Background | CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression. |
Synonyms | MS4A1, Leu-16, Bp35, B-cell marker |
Reference Data |
Documents
Product Manuals |
FAQs |
SDS |
Resources
抗体相关资料 |
Customer
Reviews
Loading...