ICAM3 Mouse Monoclonal Antibody [Clone ID: 186-2G9]
CAT#: AM50238PU-T
ICAM3 mouse monoclonal antibody, clone 186-2G9, Purified
Size: 100 ug
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CNY 3,223.00
货期*
5周
规格
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Specifications
Product Data | |
Clone Name | 186-2G9 |
Applications | FC, IF, IHC, IP, WB |
Recommend Dilution | Functional Studies: Blocks binding of CD11a (LFA-1) to CD50 (Use Azide free Antibody). ELISA: Use BSA free Antibody for Coating. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: HL-60 or THP-1 cells. Lymph node and tonsil. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Stimulated Human leukocytes. |
Specificity | Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This Monoclonal Antibopdy is excellent for staining of formalin/paraffin tissues. Cellular Localization: Cell surface. |
Formulation | 10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide |
Concentration | lot specific |
Purification | Protein A/G Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C. |
Predicted Protein Size | 110-160 kDa |
Gene Name | intercellular adhesion molecule 3 |
Database Link | |
Synonyms | ICAM-3, ICAM-R, CDw50 |
Reference Data |
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