CD68 Mouse Monoclonal Antibody [Clone ID: C68/684]

Specifications

Product Data
Clone Name	C68/684
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 0.5-1 µg/ml. Western Blotting: 0.5-1 µg/ml. Immunoprecipitation: 0.5-1 µg/500 µg protein lysate. Immunohistochemistology (Formalin-Fixed): 0.25-0.5 µg/ml for 30 minutes at RT. (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Positive Control: Tonsil, Lymph Node or Spleen.
Reactivity	Feline, Human, Monkey, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human CD68 protein
Specificity	This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules. Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	110 kDa
Gene Name	CD68 molecule
Database Link	Entrez Gene 968 Human UniProt ID P34810
Synonyms	Gp110, Macrosialin, Macrophage marker
Reference Data

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