HIF-1 alpha (HIF1A) Mouse Monoclonal Antibody [Clone ID: HIF1A-84]
CAT#: AM50284PU-S
HIF-1 alpha (HIF1A) mouse monoclonal antibody, clone HIF1A-84, Purified
Size: 20 ug
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CNY 5,606.00
货期*
5周
规格
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Specifications
Product Data | |
Clone Name | HIF1A-84 |
Applications | FC, IF |
Recommend Dilution | ELISA: For coating, order Ab without BSA. Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 0.5-1 µg/ml. Functional Studies: Use Antibody without BSA and Azide.
Positive Control: Cobalt chloride treated HeLa cells. Breast or bladder carcinomas. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Recombinant Human HIF1 alpha protein. |
Specificity | This Antibody reognizes HIF1 alpha. Cellular Localization: Nuclear and cytoplasmic. |
Formulation | 10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide |
Concentration | lot specific |
Purification | Protein A/G Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C. |
Predicted Protein Size | 92-110 kDa |
Gene Name | hypoxia inducible factor 1 alpha subunit |
Database Link | |
Background | HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions. |
Synonyms | HIF-1 alpha, Hypoxia-inducible factor 1 alpha, ARNT-interacting protein, Member of PAS protein 1, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78 |
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