PAX7 Mouse Monoclonal Antibody [Clone ID: PAX7/497]

Specifications

Product Data
Clone Name	PAX7/497
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/million cells.  Immunofluorescence: 0.5-1 µg/ml. Western Blotting: 0.5-1 µg/ml.  Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.  Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.  Positive Control: HeLa, Jurkat, A431, MCF-7, U2OS or NIH/3T3 cells. Stomach or Rhabdomyosarcoma.
Reactivity	Human, Mouse, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human PAX7 protein.
Specificity	The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell- derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveo- lar rhabdomyosarcoma). Cellular Localization: Nuclear.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	57 kDa
Gene Name	paired box 7
Database Link	Entrez Gene 5081 Human UniProt ID P23759
Background	The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).
Synonyms	Pax-7, HUP1
Reference Data

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